Ecosystems Assessments BUNDLE


Assess understanding of NGSS MS-LS2-1, MS-LS2-2, MS-LS2-3, and MS-LS2-4.

Grades 6 – 8

SAVE OVER 30% when you purchase all the ecosystems assessments you’ll need for ALL of the NGSS middle school ecology standards!

These assessments cover standards: MS-LS2-1 MS-LS2-2 MS-LS2-3 MS-LS2-4 MS-LS2-5 MS-LS-2-6 MS-ESS3-4.

Critical thinking is required… these are not your standard definition assessments! There are diagrams to analyze and questions are written to mimic standards-based tests.


  • NGSS Ecology Exam
  • Food Webs Quiz
  • Cycles of Matter in Ecosystems Quiz
  • Populations Quiz
  • Symbiosis Quiz
  • Changes in Ecosystems Quiz


NGSS Ecology Test: This middle school-level test includes 33 multiple-choice questions, many of which include simple pictures, diagrams, data, and graphs to interpret. All questions, graphs, data, etc. are at the appropriate level for a heterogeneous class of middle school students. The test is 6 pages long (3 front/back) with an additional 1-sided answer sheet. This is a middle school-level test that covers the following topics:

  • Ecosystems (biotic and abiotic factors, etc.)
  • Interactions between organisms (competition, symbiosis, etc.)
  • Energy in ecosystems (producer, consumer, etc.)
  • Food chains (food chains, energy pyramid, etc.)
  • Populations (limiting factors, carrying capacity, etc.)
  • Changes in populations (changes in resources)
  • Succession (primary and secondary)
  • Biodiversity
  • Human impact on ecosystems

Food Webs Quiz: This is a 10-question multiple-choice quiz that covers energy roles (producer, consumer, etc.), food chains, and energy pyramids. There are two versions of the quiz.

Cycles of Matter in Ecosystems Quiz: This is a 10 question multiple-choice quiz that covers the cycling of matter portion of the MS-LS2-3 standard. There are 6 questions on what goes into and what comes out of each process, as well as the organelles involved. There are 4 multiple-choice that focus on the cyclical nature of these processes in ecosystems. There are two versions of the quiz.

Populations Quiz: This 15-question multiple-choice quiz covers limiting factors, carrying capacity, and changes to populations. NGSS standard MS-LS2-1. There are two versions of the quiz.

Symbiosis Quiz: This is a 10-question multiple-choice ecology quiz that covers mutualism, commensalism, parasitism, and predator/prey relationships. Students are given examples of two real organisms and how they interact and must decide which relationship type this represents. NGSS MS-LS2-2. There are two versions of the quiz.

Changes in Ecosystems Quiz: This is a 10 question multiple choice quiz covering primary and secondary succession. NGSS MS-LS2-4. There are two versions of the quiz.

Teacher Notes:

  • All answer keys are included.
  • Please note: these ecosystems assessments are not editable.
  • Check out more resources in the ecology section of our shop!


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